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Structured Review

Eurobio lymphocyte separation medium
Lymphocyte Separation Medium, supplied by Eurobio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphocyte+separation+medium/lymphocyte+medium+separation/pmc13254722-204-12-15
Average 86 stars, based on 1 article reviews
lymphocyte separation medium - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Isolation:

Article Title: Adoptive γδ T cell therapy controls cytomegalovirus infection in preclinical transplantation models.
Article Snippet: .. PBMCs were isolated using lymphocyte separation medium (Eurobio Scientific), and stained with an antibody directed against the TCR, coupled to biotin (Miltenyi Biotec, clone REA652, diluted 1:50), followed by anti-biotin microbeads (Miltenyi Biotec). ..

Article Title: Adoptive γδ T cell therapy controls cytomegalovirus infection in preclinical transplantation models
Article Snippet: .. PBMCs were isolated using lymphocyte separation medium (Eurobio Scientific), and stained with an antibody directed against the αβTCR, coupled to biotin (Miltenyi Biotec, clone REA652, diluted 1:50), followed by anti-biotin microbeads (Miltenyi Biotec). αβ T cells were removed from PBMCs by magnetic-activated cell sorting and remaining PBMCs were cultured for 21 days to expand Vδ2 neg γδ T cells, following the DOT cell protocol , . .. Cells were cultured in OpTmizerTM CTSTM serum-free medium (GIBCO, Life Technologies) supplemented with 10% human serum, 1% Penicillin-Streptomycin (PS), and 2 mM L-glutamine (expansion medium), at a concentration of 160,000 cells/mL in the presence of the following recombinant human cytokines (all from Peprotech): 100 ng/mL interleukin 4 (IL-4), 70 ng/mL interferon γ (IFNγ), 7 ng/mL IL-21, 15 ng/mL IL-1β and an activating antibody directed against CD3 (1 μg/mL, clone OKT-3, Biolegend).

Article Title: PD-1 blockade does not enhance alloimmunization after allogeneic dendritic cell vaccination in cancer patients
Article Snippet: .. PBMCs were isolated by centrifugation density gradient with lymphocyte separation medium (Eurobio Scientific), and frozen at −150 °C in the presence of Dimethyl Sulfoxyde (Sigma Aldrich), and used for B-cell purification (EasySepTM Human B Cell Enrichment Kit, StemCell). .. Purity exceeded 95% by CD3/CD19 staining (BD Bioscience).

Article Title: Innovative photodynamic therapy using rose bengal for the treatment of human melanoma
Article Snippet: .. PBMCs were isolated by density gradient centrifugation of the blood using a lymphocyte separation medium (Eurobio, Les Ullis, France) and 50 mL Leucosep tubes (Greiner Bio One, Courtaboeuf, France). .. In round-bottom 96-well plate (Corning, Somerville, MA, USA) 100,000 PBMCs were cultured in ML10 medium composed of RPMI 1640 medium supplemented with HEPES (25 mM), non-essential amino acids (MEM 1 x ), sodium pyruvate (1 mM), 2-mercaptoethanol (50 μM), gentamicin (10 μg/mL) (Thermo Fisher Scientific, Waltham, MA, USA), and 10% of SAB (Human AB serum).

Article Title: HIV persistence in tissues on dolutegravir-based therapy is not associated with resistance mutations to dolutegravir
Article Snippet: .. PBMCs and sperm cell pellets were isolated by lymphocyte separation medium (Eurobio ref: CMSMSL01-01). ..

Article Title: HIV persistence in tissues on dolutegravir-based therapy is not associated with resistance mutations to dolutegravir.
Article Snippet: .. PBMCs and sperm cell pellets were isolated by lymphocyte separation medium (Eurobio ref: CMSMSL01-01). ..

Staining:

Article Title: Adoptive γδ T cell therapy controls cytomegalovirus infection in preclinical transplantation models.
Article Snippet: .. PBMCs were isolated using lymphocyte separation medium (Eurobio Scientific), and stained with an antibody directed against the TCR, coupled to biotin (Miltenyi Biotec, clone REA652, diluted 1:50), followed by anti-biotin microbeads (Miltenyi Biotec). ..

Article Title: Adoptive γδ T cell therapy controls cytomegalovirus infection in preclinical transplantation models
Article Snippet: .. PBMCs were isolated using lymphocyte separation medium (Eurobio Scientific), and stained with an antibody directed against the αβTCR, coupled to biotin (Miltenyi Biotec, clone REA652, diluted 1:50), followed by anti-biotin microbeads (Miltenyi Biotec). αβ T cells were removed from PBMCs by magnetic-activated cell sorting and remaining PBMCs were cultured for 21 days to expand Vδ2 neg γδ T cells, following the DOT cell protocol , . .. Cells were cultured in OpTmizerTM CTSTM serum-free medium (GIBCO, Life Technologies) supplemented with 10% human serum, 1% Penicillin-Streptomycin (PS), and 2 mM L-glutamine (expansion medium), at a concentration of 160,000 cells/mL in the presence of the following recombinant human cytokines (all from Peprotech): 100 ng/mL interleukin 4 (IL-4), 70 ng/mL interferon γ (IFNγ), 7 ng/mL IL-21, 15 ng/mL IL-1β and an activating antibody directed against CD3 (1 μg/mL, clone OKT-3, Biolegend).

FACS:

Article Title: Adoptive γδ T cell therapy controls cytomegalovirus infection in preclinical transplantation models
Article Snippet: .. PBMCs were isolated using lymphocyte separation medium (Eurobio Scientific), and stained with an antibody directed against the αβTCR, coupled to biotin (Miltenyi Biotec, clone REA652, diluted 1:50), followed by anti-biotin microbeads (Miltenyi Biotec). αβ T cells were removed from PBMCs by magnetic-activated cell sorting and remaining PBMCs were cultured for 21 days to expand Vδ2 neg γδ T cells, following the DOT cell protocol , . .. Cells were cultured in OpTmizerTM CTSTM serum-free medium (GIBCO, Life Technologies) supplemented with 10% human serum, 1% Penicillin-Streptomycin (PS), and 2 mM L-glutamine (expansion medium), at a concentration of 160,000 cells/mL in the presence of the following recombinant human cytokines (all from Peprotech): 100 ng/mL interleukin 4 (IL-4), 70 ng/mL interferon γ (IFNγ), 7 ng/mL IL-21, 15 ng/mL IL-1β and an activating antibody directed against CD3 (1 μg/mL, clone OKT-3, Biolegend).

Cell Culture:

Article Title: Adoptive γδ T cell therapy controls cytomegalovirus infection in preclinical transplantation models
Article Snippet: .. PBMCs were isolated using lymphocyte separation medium (Eurobio Scientific), and stained with an antibody directed against the αβTCR, coupled to biotin (Miltenyi Biotec, clone REA652, diluted 1:50), followed by anti-biotin microbeads (Miltenyi Biotec). αβ T cells were removed from PBMCs by magnetic-activated cell sorting and remaining PBMCs were cultured for 21 days to expand Vδ2 neg γδ T cells, following the DOT cell protocol , . .. Cells were cultured in OpTmizerTM CTSTM serum-free medium (GIBCO, Life Technologies) supplemented with 10% human serum, 1% Penicillin-Streptomycin (PS), and 2 mM L-glutamine (expansion medium), at a concentration of 160,000 cells/mL in the presence of the following recombinant human cytokines (all from Peprotech): 100 ng/mL interleukin 4 (IL-4), 70 ng/mL interferon γ (IFNγ), 7 ng/mL IL-21, 15 ng/mL IL-1β and an activating antibody directed against CD3 (1 μg/mL, clone OKT-3, Biolegend).

Centrifugation:

Article Title: PD-1 blockade does not enhance alloimmunization after allogeneic dendritic cell vaccination in cancer patients
Article Snippet: .. PBMCs were isolated by centrifugation density gradient with lymphocyte separation medium (Eurobio Scientific), and frozen at −150 °C in the presence of Dimethyl Sulfoxyde (Sigma Aldrich), and used for B-cell purification (EasySepTM Human B Cell Enrichment Kit, StemCell). .. Purity exceeded 95% by CD3/CD19 staining (BD Bioscience).

Purification:

Article Title: PD-1 blockade does not enhance alloimmunization after allogeneic dendritic cell vaccination in cancer patients
Article Snippet: .. PBMCs were isolated by centrifugation density gradient with lymphocyte separation medium (Eurobio Scientific), and frozen at −150 °C in the presence of Dimethyl Sulfoxyde (Sigma Aldrich), and used for B-cell purification (EasySepTM Human B Cell Enrichment Kit, StemCell). .. Purity exceeded 95% by CD3/CD19 staining (BD Bioscience).

Gradient Centrifugation:

Article Title: Innovative photodynamic therapy using rose bengal for the treatment of human melanoma
Article Snippet: .. PBMCs were isolated by density gradient centrifugation of the blood using a lymphocyte separation medium (Eurobio, Les Ullis, France) and 50 mL Leucosep tubes (Greiner Bio One, Courtaboeuf, France). .. In round-bottom 96-well plate (Corning, Somerville, MA, USA) 100,000 PBMCs were cultured in ML10 medium composed of RPMI 1640 medium supplemented with HEPES (25 mM), non-essential amino acids (MEM 1 x ), sodium pyruvate (1 mM), 2-mercaptoethanol (50 μM), gentamicin (10 μg/mL) (Thermo Fisher Scientific, Waltham, MA, USA), and 10% of SAB (Human AB serum).



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